Pressure cooker as autoclaveEarly WIP

Safety consideration

Pressure vessels are dangerous. Steam can scald. Even though modern pressure cooker have redudant pressure release mecanism, messing with the regulator on a pressure cooker carries a risk.

Using a pressure cooker for sterilizing solution for (own) human use obviously has a worse risk profile that industrially manufactured products. This page contains tips to minimize the risk increase, but far from negates it. Whether this is right for you depend on your risk tolerance - it is a personal decision you must seriously ponder. Please do not pass the fruit of this sort of protocol to other people that may not realize the implied risk.

And, as always, please think through before acting.

Choice of pressure cooker model

Due to the EU directive on pressure vessels, pressure cookers in the EU are not marketed for canning/sterilization capability, even though they may be adequate for that use. There is no such limitation in the USA. It is also possible to import a pressure cooker, or downright stove top autoclave, though shipping will be expensive (weight).

Some manufacturer have the same model available in both markets, so finding a suitable pressure cooker in the EU may necessitate finding such model, and checking for identical part number for the vessel and essential components.

Non-exhaustive list of suitable model:

(as manufacturer may change individual parts without changing the model name, still check part number matching)

Modifications

Shimming the main pressure regulator valve

Sample holder basket setup

Consumables

Water

Secondary containers

Indicators

(see also: routine validation)

Protocol of operation

Dry air elimination

Most important step to have an actually sterilizing setup.

Duration

End of cycle for closed container

Validation

Physico-chemical initial validation

Biological initial validation

Media fill

Replacement for fastidous spore forming bacteria

Thermophile culture is most annoying, but can be avoided for a limited increase in risk.

Routine validation

Troubleshooting

Misc